Bionic Apparatus For Fluorescent Light Estimation

Overview

Here you can find the instructions for assembling a low cost and open source opto-mechanical device with the aim of acquiring multi-channel time-lapse images of bacterial colonies growing in agar plates.

This device uses a system of addressable LEDs and fluorescence filters to estimate the spatio-temporal distribution of different fluorescently-tagged sub-populations from time-lapse images obtained using a standard DSLR camera with a macro lens.

Getting Started

You can see the full project in this manuscript [link].

The repository with hardware design files, code and materials can be found here.

Prerequisites

To assemble this device you need access to a laser cutter and a 3D printer. You also need a PC and a digital camera. The complete list of materials, electronics and parts can be found here.

You will also need the following tools:

• 3mm Allen Key
  
• Screw drivers
  
• Soldering station
  
• Cutting pliers
  
• Wire stripper
  
• Multimeter
  

Authors

Systems Biology Lab, CCG-UNAM

Acknowledgements

We thank the help and input from past and present members of the Systems and Synthetic Laboratory at CCG-UNAM.

MIT License

Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions:

The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software.

THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE.